Anti-Human CA9 Recombinant Antibody (MSC3)

Customer Review

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Michael Lee
22.Aug,23

Excellent for CA9 Studies

The Anti-CA9 Antibody (MSC3) has been excellent for our CA9 detection assays. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Laura Young
19.Dec,22

Effective and Dependable

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CA9 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

David Clark
07.Oct,21

Consistent High-Quality Results

This antibody has proven to be consistent and dependable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for CA9 have been particularly beneficial.

Q&As

1. Can the anti-CA9 antibody MSC3 be used in immunohistochemistry on paraffin-embedded tissue sections?

   A: Yes, the anti-CA9 antibody MSC3 (TAB-1458CL) is suitable for use in immunohistochemistry on paraffin-embedded tissue sections. It provides specific staining of CA9, allowing for detailed analysis of its expression in tissue samples.

2. What is the optimal dilution for using the anti-CA9 antibody MSC3 in Western blot?

   A: The optimal dilution for using the anti-CA9 antibody MSC3 (TAB-1458CL) in Western blot is typically 1:500 to 1:2000. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

3. Is this anti-CA9 antibody MSC3 effective in ELISA applications?

   A: Yes, the anti-CA9 antibody MSC3 (TAB-1458CL) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of CA9.

4. Can the anti-CA9 antibody MSC3 be used in flow cytometry to analyze CA9 expression?

   A: Yes, the anti-CA9 antibody MSC3 (TAB-1458CL) can be used in flow cytometry to analyze CA9 expression. It provides specific binding to CA9, allowing for the detection of CA9-expressing cells in flow cytometry assays.

5. What are the recommended storage conditions for the anti-CA9 antibody MSC3?

   A: The recommended storage condition for the anti-CA9 antibody MSC3 (TAB-1458CL) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Boreel, Daan F., et al. "Quantitative Imaging of Hypoxic CAIX-Positive Tumor Areas with Low Immune Cell Infiltration in Syngeneic Mouse Tumor Models." Molecular Pharmaceutics 20.4 (2023): 2245-2255. https://doi.org/10.1021/acs.molpharmaceut.3c00045
   This study investigates the quantitative imaging of hypoxic CAIX-positive tumor areas with low immune cell infiltration in syngeneic mouse tumor models. The research focuses on developing a radiolabeled antibody targeting murine CAIX to visualize chronic hypoxia in these models and study the immune cell population in these hypoxic areas. Using the anti-mCAIX antibody (MSC3) conjugated to diethylenetriaminepentaacetic acid (DTPA) and radiolabeled with indium-111 ([111In]In-MSC3), the study demonstrated that this tracer specifically binds to CAIX-expressing cells in vitro and accumulates in CAIX-positive areas in vivo. The study highlighted that these CAIX-positive areas are characterized by reduced infiltration of immune cells, underscoring the immunosuppressive nature of chronic hypoxia in tumors. The findings suggest that this imaging technique can be used to optimize immuno- and radiotherapy efficacy by visualizing CAIX expression before or during hypoxia-targeted treatments.
   Creative Biolabs provided the crucial human IgG1 anti-mouse CAIX antibody (Cat# TAB-1458CL) for this research. This antibody was pivotal in enabling the conjugation and radiolabeling processes necessary for developing the [111In]In-MSC3 tracer. By facilitating specific binding to CAIX-expressing cells and accumulation in hypoxic tumor regions, the MSC3 antibody allowed for the effective visualization and analysis of CAIX-positive hypoxic areas. This contribution was instrumental in advancing the study's objective of using CAIX as a biomarker for chronic hypoxia and its associated immunosuppressive microenvironment in preclinical models.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-1458CL, RRID: AB_3111834)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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